Mass spectrometry (MS)-based ubiquitinomics allows a system-level understanding of ubiquitin signalling.
In this publication, we focus on:
a background to ubiquitinome profiling
presentation of a scalable and robust workflow for deep and precise in vivo ubiquitinome profiling using DIA-MS (data-independent acquisition mass spectrometry) and neural network based data processing
comprehensive mapping of substrates of deubiquitinase USP7, an anticancer drug target known to regulate tumour suppressor p53
application of the method including rapid mode of action profiling of candidate drugs targeting deubiquitinases or ubiquitin ligases