Science Pool

Time-Resolved Ubiquitinome Profiling by DIA-MS Reveals USP7 Targets

Mass spectrometry (MS)-based ubiquitinomics allows a system-level understanding of ubiquitin signalling. 

In this publication, we focus on:

  • a background to ubiquitinome profiling
  • presentation of a scalable and robust workflow for deep and precise in vivo ubiquitinome profiling using DIA-MS (data-independent acquisition mass spectrometry) and neural network based data processing
  • comprehensive mapping of substrates of deubiquitinase USP7, an anticancer drug target known to regulate tumour suppressor p53
  • application of the method including rapid mode of action profiling of candidate drugs targeting deubiquitinases or ubiquitin ligases